The RA's gone off on a short but well-earned trip to the sun, and before she left she made the H. influenzae hfq knockout I've been waiting for. So yesterday, after clicking 'Submit' on our NSERC grant proposal, I did competence time courses of wildtype cells and the mutant, hoping to see a difference in transformation frequencies (preferably a decrease).
You can read all the background in this post from last March. And here's a hexameric structure for Hfq (from Wikipedia):
The colonies are still too tiny to count, but it looks like the transformation frequency is down about 10-fold in the mutant!
Next step, make some DNA from the mutant so I can introduce the mutation into my hypercompetent mutant backgrounds.
Clik here to view.
Image may be NSFW.
Clik here to view.
You can read all the background in this post from last March. And here's a hexameric structure for Hfq (from Wikipedia):
The colonies are still too tiny to count, but it looks like the transformation frequency is down about 10-fold in the mutant!
Next step, make some DNA from the mutant so I can introduce the mutation into my hypercompetent mutant backgrounds.
Image may be NSFW.
Clik here to view.
Clik here to view.
Image may be NSFW.
Clik here to view. Image may be NSFW.
Clik here to view. Image may be NSFW.
Clik here to view. Image may be NSFW.
Clik here to view. Image may be NSFW.
Clik here to view. Image may be NSFW.
Clik here to view. Image may be NSFW.
Clik here to view.
Image may be NSFW.Clik here to view.
Image may be NSFW.Clik here to view.
Image may be NSFW.Clik here to view.
Image may be NSFW.Clik here to view.
Image may be NSFW.Clik here to view.
Image may be NSFW.Clik here to view.
Image may be NSFW.Clik here to view.
Clik here to view.
Image may be NSFW.Clik here to view.
