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Here's the gel photo


These DNAs were all stored in the fridge (4 °C) in aqueous solution (10 mM Tris 1 mM EDTA pH 8.0) for two months before this gel was run.  The DNAs in the 'ss' lanes were heated to 95°C for 10 min before loading to separate the strands and reveal the effects of any single-strand breaks.

These DNAs show no sign of degradation; compare to the original photo here.  In particular, the DNA fragments from cells grown with limiting phosphate and 40 mM arsenate are actually slightly longer than the fragments from cells grown with limiting phosphate and no arsenate.  (I don't think this difference is significant; the important point is that the fragments aren't any shorter.)

Because these large fragments typically migrate at the resolving limit of the gel, all I can say with confidence is that the fragments in all four preps are all significantly larger than 30 kb.  This is the size range we expect for chromosomal DNA in a normal DNA prep.  I don't have size standards for single-stranded DNA (I should have heated the lambda fragments but forgot to) so all I can say about the length distribution of single strands is that the four preps are all very similar.

This result tells is that DNAs from arsenate-grown cells are not undergoing degradation in storage due to slow hydrolysis of arsenate diester bonds in the DNA backbone, as suggested by an earlier anonymous commenter.
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